Yakae-Prajamduen-Jamod recipe reduced anxiety behaviorand brain oxidative damage in ovariectomized mice

Yakae-Prajamduen-Jamod (ABP), which was acquired from Chao Phraya Abhaibhubejhr Hospital, is a traditional Thai herbal formula to treat perimenopausal and menopausal symptoms. However, its mechanism remains unknown. The present study aimed to investigate the effects of ABP on ovariectomy-induced anxiety and oxidative stress in mice. Ovariectomized mice exhibited not only anxiety in elevated plus maze and mirror chamber tests, but also brain oxidative stress when compared with control. Ovariectomy-induced behavioral and neurochemical alterations were attenuated by ABP treatment (500 mg/kg/day). We also analyzed antioxidant activities of ABP using DPPH and ABTS assays. ABP showed radical scavenging activity with IC50 58.59 and 44.99 µg/mL, respectively. Total phenolic and flavonoid contents were 110.53±1.50 mg GAE and 152.25±0.5 mg rutin equivalents per gram extract, respectively. These findings suggest that ABP attenuated the ovariectomy-induced anxiety and oxidative brain injury via antioxidative properties. Therefore, ABP is an alternative choice of anxiety disorder in the menopausal transition.