IRF7
生物
干扰素
基因亚型
干扰素调节因子
增强子
病毒学
选择性拼接
新城疫
先天免疫系统
分子生物学
病毒
细胞生物学
遗传学
基因
转录因子
受体
作者
Yu-chen MA,Hua-yuan CHEN,Shen-yan GAO,Xiao-zhan ZHANG,Yong-tao LI,Xinglun Yang,Jun Zhao,Zeng WANG
标识
DOI:10.1016/j.jia.2022.12.015
摘要
Type I interferon (IFN-I) provides an important first line to protect avian species against pathogens invasion. IFN regulatory factor 7 (IRF7) has been identified as the most important regulator for both DNA and RNA virus-induced IFN-I production in chickens. Although four splicing variants of IRF7 have been identified in mammals, it is still unclear whether alternative splicing patterns and the function of IRF7 isoform(s) exist in chickens. In this study, we reported a novel short transcript isoform of chicken IRF7 (chIRF7), termed chIRF7-iso, which contained an intact N-terminal DNA-binding domain (DBD) and 14 amino acids different from chIRF7 in the C-terminal. Overexpression of chIRF7 in chicken leghorn male hepatocellular (LMH) cells activated the IFN-β promoter and significantly inhibited Newcastle disease virus (NDV) and fowl adenovirus serotype 4 (FAdV-4) replication. Conversely, overexpression of chIRF7-iso blocked the IFN-β promoter activity and was favorable for NDV and FAdV-4 replication in vitro. Collectively, our results confirm that a novel chIRF7 isoform-mediated negative regulates IFN-β production, which will contribute to understanding the role of chIRF7 in innate antiviral response in chicken.
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