神经保护
自噬
精液
β淀粉样蛋白
体内
细胞凋亡
生物
淀粉样蛋白(真菌学)
程序性细胞死亡
药理学
生物化学
遗传学
肽
植物
作者
Yong Tang,Jing Wei,Xiaofang Wang,Tao Long,Xiaohong Xiang,Liqun Qu,Xingxia Wang,Chong‐Lin Yu,Xue Xiao,Xiaobin Hu,Jing Zen,Qin Xu,Anguo Wu,Jianming Wu,Da‐Lian Qin,Xiaogang Zhou,Betty Yuen‐Kwan Law
标识
DOI:10.4103/nrr.nrr-d-23-00954
摘要
JOURNAL/nrgr/04.03/01300535-202419110-00027/figure1/v/2024-03-08T184507Z/r/image-tiff Amyloid-beta-induced neuronal cell death contributes to cognitive decline in Alzheimer's disease. Citri Reticulatae Semen has diverse beneficial effects on neurodegenerative diseases, including Parkinson's and Huntington's diseases, however, the effect of Citri Reticulatae Semen on Alzheimer's disease remains unelucidated. In the current study, the anti-apoptotic and autophagic roles of Citri Reticulatae Semen extract on amyloid-beta-induced apoptosis in PC12 cells were first investigated. Citri Reticulatae Semen extract protected PC12 cells from amyloid-beta-induced apoptosis by attenuating the Bax/Bcl-2 ratio via activation of autophagy. In addition, Citri Reticulatae Semen extract was confirmed to bind amyloid-beta as revealed by biolayer interferometry in vitro, and suppress amyloid-beta-induced pathology such as paralysis, in a transgenic Caenorhabditis elegans in vivo model. Moreover, genetically defective Caenorhabditis elegans further confirmed that the neuroprotective effect of Citri Reticulatae Semen extract was autophagy-dependent. Most importantly, Citri Reticulatae Semen extract was confirmed to improve cognitive impairment, neuronal injury and amyloid-beta burden in 3×Tg Alzheimer's disease mice. As revealed by both in vitro and in vivo models, these results suggest that Citri Reticulatae Semen extract is a potential natural therapeutic agent for Alzheimer's disease via its neuroprotective autophagic effects.
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