同种类的
皮质神经元
神经科学
生物神经网络
细胞因子
钙显像
体内
生物
细胞生物学
化学
钙
免疫学
物理
热力学
生物技术
有机化学
作者
Mamoru Sakaibara,Hideaki Yamamoto,Hakuba Murota,Nobuyuki Monma,Shusei Sato,Ayumi Hirano‐Iwata
标识
DOI:10.1016/j.bbrc.2023.149379
摘要
Cortical neurons in dissociated cultures are an indispensable model system for pharmacological research that provides insights into chemical responses in well-defined environments. However, cortical neurons plated on homogeneous substrates develop an unstructured network that exhibits excessively synchronized activity, which occasionally masks the consequences induced by external substances. Here, we show that hyperactivity and excessive synchrony in cultured cortical networks can be effectively suppressed by growing neurons in microfluidic devices. These devices feature a hierarchically modular design that resembles the in vivo network. We focused on interleukin-6, a pro-inflammatory cytokine, and assessed its acute and chronic effects. Fluorescence calcium imaging of spontaneous neural activity for up to 20 days of culture showed detectable modulation of collective activity events and neural correlation in micropatterned neurons, which was not apparent in neurons cultured on homogeneous substrates. Our results indicate that engineered neuronal networks provide a unique platform for detecting and understanding the fundamental effects of biochemical compounds on neuronal networks.
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