炎症
小胶质细胞
脊髓损伤
巨噬细胞极化
SOCS3
细胞生物学
细胞凋亡
转染
M2巨噬细胞
下调和上调
信号转导
巨噬细胞
免疫学
生物
医学
神经科学
车站3
脊髓
细胞培养
体外
基因
生物化学
遗传学
作者
Jie Ren,Bin Zhu,Guangjin Gu,Wencan Zhang,Junjin Li,Hongda Wang,Min Wang,Xiaomeng Song,Zhijian Wei,Shiqing Feng
标识
DOI:10.1038/s41419-023-05607-4
摘要
Macrophage/microglia polarization acts as an important part in regulating inflammatory responses in spinal cord injury (SCI). However, the regulation of inflammation of Schwann cell-derived exosomes (SCDEs) for SCI repair is still unclear. Therefore, we intend to find out the effect of SCDEs on regulating the inflammation related to macrophage polarization during the recovery of SCI. Firstly, the thesis demonstrated that SCDEs could attenuate the LPS- inflammation in BMDMs by suppressing M1 polarization and stimulating M2 polarization. Similarly, SCDEs improved functional recovery of female Wistar rats of the SCI contusion model according to BBB (Basso, Beattie, and Bresnahan) score, electrophysiological assay, and the gait analysis system of CatWalk XT. Moreover, MFG-E8 was verified as the main component of SCDEs to improve the inflammatory response by proteomic sequencing and lentiviral transfection. Improvement of the inflammatory microenvironment also inhibited neuronal apoptosis. The knockout of MFG-E8 in SCs can reverse the anti-inflammatory effects of SCDEs treatment. The SOCS3/STAT3 signaling pathway was identified to participate in upregulating M2 polarization induced by MFG-E8. In conclusion, our findings will enrich the mechanism of SCDEs in repairing SCI and provide potential applications and new insights for the clinical translation of SCDEs treatment for SCI.
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