Isolation of Volatiles in Honeysuckle by Multiple Approaches with Determination by Gas Chromatography–Mass Spectrometry (GC–MS) and Headspace Solid-Phase Microextraction-Gas Chromatography–Mass Spectrometry (HS-SPME/GC–MS)

The volatile components of honeysuckle were determined using hydro-distillation (HD), water extraction coupling rectification (WER), and water extraction coupling rectification with solvent extraction (WER-SE). Gas chromatography–mass spectrometry (GC–MS) and headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME/GC–MS) identified 127 compounds. There were 27 common compounds extracted by these methods. A heatmap of the 27 common compounds indicates that WER-SE and WER were complementary approaches. In addition, 59 compounds were detected for the first time in honeysuckle. The results of this study provide a reference for the determination of volatile components in plants.