诱导多能干细胞
再生医学
细胞生物学
细胞培养
人诱导多能干细胞
细胞分化
生物
细胞
细胞粘附
电池类型
干细胞
表型
胚胎干细胞
遗传学
基因
作者
Lei Yu,Junjun Li,Itsunari Minami,Xiaodong Qu,Shigeru Miyagawa,Nozomu Fujimoto,Kouichi Hasegawa,Yong Chen,Yoshiki Sawa,Hidetoshi Kotera,Li Liu
标识
DOI:10.1002/adhm.201900165
摘要
Abstract Human pluripotent stem cells (hPSCs) have been widely used for various applications including disease modeling and regenerative medicine, among others. Recently, an increasing number of studies has focused on heterogeneity among hPSCs, which could affect cell quality and subsequent applications. In this study, a nanofibrous platform is developed for single human induced pluripotent stem cell isolation and culture. One type of single cell‐derived subclone is established and found to have a distinct morphology compared to other subclones. When used for differentiation toward cardiomyocytes, this type of subclone demonstrates higher differentiation efficiency, increased maturation, and stronger beating compared to those derived from the other subclones. The findings provide a convenient method for single‐cell isolation and culture, and demonstrate that variations in differentiation tendencies exist among subclones from the same cell line. This substrate adhesion‐based selection process could be used to obtain cell lines with improved differentiation efficiency toward cardiomyocytes and other cell types, which would be advantageous for studies in various fields.
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