Aptamer-based Homogeneous Analysis for Food Control

Background: Highly sensitive and rapid analysis of food contaminants is of great significance for food safety control. Aptamer is a new kind of recognition molecules which could be applied for constructing homogeneous analysis assays, potentially achieving highly sensitive, cheap and rapid profiling of food contaminants. Methods: An overview of the literature concerning the homogeneous analysis of food contaminations based on aptamers has been reviewed (focused on the most recent literature, 2000-2018). Results: Attributed to aptamer’s controllability, designability and feasibility for the adoption of nucleic acid amplification, rapid, highly sensitive homogeneous assay for various food contaminants could be constructed. The structure-switching aptamer probe would confer quick, efficient and specific response to target food contaminants. Besides, the capability of amplification of aptamer sequences or nucleic acid probes would lead to highly sensitive detection. Conclusion: Aptamer-based homogeneous analysis methods have already been applied to detect various food contaminations ranging from toxins, heavy metal and pesticide to allergen and pathogenic bacteria. However, it is still a challenge to achieve robust and accurate detection of food contaminants in complex food samples.