化学
对接(动物)
淀粉酶
类黄酮
橙皮素
结合位点
荧光光谱法
酶
芦丁
立体化学
槲皮素
阿卡波糖
活动站点
没食子酸表没食子酸酯
儿茶素
木犀草素
生物化学
荧光
多酚
护理部
物理
医学
抗氧化剂
量子力学
作者
Alejandra I. Martinez-Gonzalez,Ángel G. Díaz‐Sánchez,Laura A. de la Rosa,Ismael Bustos‐Jaimes,Emilio ́Álvarez-Parrilla
标识
DOI:10.1016/j.saa.2018.08.057
摘要
Flavonoids are recognized to regulate animals' food digestion processes trough interaction with digestive enzymes. The binding capacity of hesperetin (HES), luteolin (LUT), quercetin (QUE), catechin (CAT) and rutin (RUT) with pancreatic α-amylase were evaluated, using UV–Vis spectroscopy, fluorescence and molecular docking. Using p-nitrophenyl-α-d-maltopentoside (pNPG5) as substrate analog, LUT showed the best inhibitory capacity, even better than that of the positive control, acarbose (ACA). A mixed-type inhibition was observed for HES, LUT and QUE, a competitive-type for ACA, while no inhibition was observed with CAT and RUT. In agreement with kinetic results, α-amylase presented a higher affinity for LUT, when analyzed by fluorescence quenching. The binding of flavonoids to amylase followed a static mechanism, where the binding of one flavonoid per enzyme molecule was observed. Docking analysis showed that flavonoids bound near to enzyme active site, while ACA bound in another site behind the catalytic triad. Extrinsic fluorescence analysis, together with docking analysis pointed out that hydrophobic interactions regulated the flavonoid-α-amylase interactions. The present study provides evidence to understand the relationship of flavonoids structure with their inhibition mechanism.
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