CTGF公司
基质金属蛋白酶
纤维化
马森三色染色
转化生长因子
污渍
克罗恩病
病理
染色
H&E染色
化学
医学
内科学
生长因子
疾病
生物化学
受体
基因
作者
Hui Wang,Zhe Yang,Kang Chen,Ruilian Yu,Li Chong Xu,Gaohong Lv
标识
DOI:10.1016/j.jff.2021.104875
摘要
We aim at exploring the potential therapeutic effect and mechanisms of artificial Isaria cicadae Miquel (IcM) in Crohn’s disease (CD)-related intestinal fibrosis. The Crohn's fibrosis model was induced by Trinitrobenzene sulphonic acid (TNBS). Mice were divided into control group, TNBS control group, and IcM treatment group (H, M, D). After four weeks, the entire length of the colon and serum were collected, and Hematoxylin and Eosin staining and Masson’s collagen trichrome staining were performed for determining the colon histological score. The contents of TGF-β1, CTGF, and IFN-γ were determined by ELISA. FN and α-SAM were evaluated by Western blotting; further, MMP-1, MMP-3, and TIMP-1 mRNA were determined by qRT-PCR. We observed that IcM prevents intestinal fibrosis in mice with CD. In addition, IcM was capable of significantly down-regulating the expression of major fibrosis markers (⍺-SMA and fiber-fibroin) and key fibrogenic molecules TGF-β1 and CTGF, and promoting the expression of IFN-γ, which has antifibrotic effects to maintain MMP/TIMP balance.
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