巨噬细胞
背景(考古学)
先天免疫系统
骨髓
体内
败血症
细胞生物学
生物
免疫系统
体外
免疫学
遗传学
古生物学
作者
Tivoli Nguyen,Jie Du,Yan Chun Li
出处
期刊:STAR protocols
[Elsevier]
日期:2021-12-01
卷期号:2 (4): 101004-101004
被引量:14
标识
DOI:10.1016/j.xpro.2021.101004
摘要
Macrophages are key innate immune cells involved in a broad spectrum of physiological and pathological processes. Macrophage depletion with clodronate-liposomes is commonly used to investigate in vivo functions of macrophages in mice. Here, we describe a protocol that combines the depletion of resident macrophages with the reconstitution of the mice with in vitro differentiated, lentivirus-transduced bone marrow-derived macrophages (BMDMs) in the context of an experimental sepsis model. This experimental strategy is easily adapted to other experimental designs. For complete details on the use and execution of this protocol, please refer to Du et al. (2020).
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