Application of the in-situ biological detoxification polymer for the improvement of AFB1 detoxification

A novel biological detoxification polymer (BDP) was in-situ synthesis by the co-immobilization of the compound enzymes (laccase and Aspergillus niger crude enzyme) and the aminated mesoporous silica nanoparticles (MSN-NH2) with zeolitic imidazolate framework-8 (ZIF-8) for the detoxification of aflatoxin B1 (AFB1). The ZIF-8 shell of BDP acted as the AFB1 adsorbent around the compound enzymes inside the BDP. The detoxification rate of BDP at 35 °C for 24 h was 96.7%. The detoxification of AFB1 with the added mass ratio of BDP 1% to moldy corn was 65.8%. After the BDP was stored at 4 °C for 30 d, the enzyme activity remained 70.9%. Moreover, the enzyme activity and detoxification rate with BDP remained 80.7% and 80.0%, respectively, after the fourth repeated detoxification. The compound enzymes acted on the C–O bond and methoxyl group in the side chain of AFB1. Three degradation products (C16H16O5, C16H14O4, C15H14O2) with different structures were detected in the degradation of AFB1 with the BDP. Application of the in-situ BDP represents a promising way to improve the detoxification of AFB1.