甘油
1,3-丙二醇
化学
罗伊乳杆菌
产量(工程)
食品科学
发酵
生物化学
材料科学
冶金
乳酸菌
作者
Yuanze Sun,Noemi Gutierrez‐Maddox,Anthony N. Mutukumira,I. S. Maddox,Shu Qin
出处
期刊:Fermentation
[MDPI AG]
日期:2022-05-16
卷期号:8 (5): 227-227
被引量:3
标识
DOI:10.3390/fermentation8050227
摘要
Limosilactobacillus reuteri strains can secrete a potentially valuable chemical and broad-spectrum antimicrobial substance named reuterin (3-hydroxypropionaldehyde, 3-HPA). L. reuteri DPC16 is a novel and patented probiotic strain that is used commercially because of its proven ability to kill various foodborne pathogens. A two-step process has been developed for reuterin production from glycerol using L. reuteri DPC16. Cells were grown, followed by harvesting, and then were incubated with glycerol for reuterin production. Parameters investigated during the glycerol conversion included the initial glycerol concentration, the biomass concentration, pH, culture age at harvesting, conversion time, and temperature. The highest reuterin yield was obtained using 21 g/L 24 h old cells, to convert glycerol solution (300 mmol/L) in 1 h at 30 °C and pH 6.2. The most efficient transformation of glycerol to reuterin was achieved in approximately 20 h of growth of cells at 25 °C and pH 6.8. Using the regression equation of this study, the maximum concentration of reuterin can be obtained using 25 g/L 20 h old DPC6 cells to ferment 350 mmol/L glycerol (initial concentration) for 2 h at 25 °C and pH 6.8 The ranking of effects on reuterin production for the six single factors was glycerol concentration > pH > conversion time > biomass concentration > temperature > culture age.
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