Microsatellite markers in the traditional Chinese medicinal herb Gynostemma pentaphyllum (Cucurbitaceae)

• Premise of the study: Microsatellite primers were developed for Gynostemma pentaphyllum , a traditional Chinese medicinal herb, to investigate its population genetic diversity. • Methods and Results: Using the Microsatellite Sequence Enrichment protocol, 14 polymorphic primers sets were identified in four Chinese Gynostemma pentaphyllum populations. The primers amplified di‐ and trinucleotide repeats with 1–6 alleles per locus, and the observed heterozygosities ranged from 0.000 to 1.000 per population. • Conclusions: These markers will facilitate further studies on genetic structure and gene flow of Gynostemma pentaphyllum populations.