乳酸链球菌素
金黄色葡萄球菌
化学
细菌生长
最小抑制浓度
微生物学
食品科学
细菌
生物化学
生物
抗菌剂
体外
有机化学
遗传学
作者
Xiaohong Wang,Ying Liu,Bijun Xie,Xianming Shi,Zhou Junchu,Honglin Zhang
标识
DOI:10.1002/mnfr.200400079
摘要
A novel microcalorimetric technique based on the bacterial heat output was applied to evaluate the biological effect of nisin on the growth of Staphylococcus aureus. The thermogenic curves of S. aureus in the presence of nisin were studied by an LKB-2277 Thermal Activity Monitor. The thermokinetic parameters, such as the growth rate constant (k), the generation times (G), the inhibitory ratio (I), and the half inhibitory concentration (IC50), for the growth of S. aureus at different nisin concentrations were determined. The relationship between the growth rate constant (k) and the concentration of nisin (c) is nearly linear, which can be modeled by the formula k = 0.03794 - 4.005 x 10(-4) x c, with a correlation coefficient of -0.9971. Based on this model, we obtained the critical inhibitory concentration of nisin on the growth of S. aureus at 94.73 IU/mL. We proposed that this microcalorimetric method could be a useful tool in monitoring the biological effect of nisin on microorganisms, and providing valuable information on the study of microorganism metabolisms.
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