油棕
生物
灵芝
链霉菌
菌丝体
琼脂平板
琼脂
孢子
茎腐病
拉伤
园艺
植物
棕榈油
微生物学
食品科学
细菌
灵芝
解剖
遗传学
作者
A B Nur Azura,Mashitah M. Yusoff,Geok Yuan Annie Tan,Jegadeesh Raman,David R. Appleton,Vikineswary Sabaratnam
出处
期刊:Journal of Industrial Microbiology & Biotechnology
[Oxford University Press]
日期:2016-04-01
卷期号:43 (4): 485-493
被引量:17
标识
DOI:10.1007/s10295-015-1724-4
摘要
Abstract Actinomycete strain AUM 00500 was 99.5 % similar to Streptomyces sanglieri NBRC 100784T and was evaluated for antagonistic activity towards Ganoderma boninense, the causative fungus of basal stem rot of oil palm. The strain showed strong antifungal activity towards G. boninense in in vitro and SEM analysis showed various modes of inhibition of the fungus. Ethyl acetate extracts of single culture and inhibition zone of cross-plug culture by HPLC indicated that strain AUM 00500 produced two different antibiotics of the glutarimide group namely cycloheximide and actiphenol. In greenhouse trials, oil palm seed treated with spores of S. sanglieri strain AUM 00500 at 109 cfu/ml showed significant (P < 0.05) increase in oil palm seedlings growth when compared to the control. Streptomyces sanglieri strain AUM 00500 successfully colonised the epidermal surface of the roots of treated oil palm seedlings and it was recovered from root fragments plated on starch casein agar.
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