亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Network pharmacology and pharmacokinetics integrated strategy to investigate the pharmacological mechanism of Xianglian pill on ulcerative colitis

溃疡性结肠炎 药代动力学 药理学 黄连碱 吴茱萸碱 医学 小桶 小檗碱 内科学 巴马汀 化学 疾病 基因表达 基因本体论 基因 生物化学
作者
Chang‐Shun Liu,Ting Xia,Zhen‐Ye Luo,Yuanyuan Wu,Yan‐Nan Hu,Feilong Chen,Qingfa Tang,Xiaomei Tan
出处
期刊:Phytomedicine [Elsevier BV]
卷期号:82: 153458-153458 被引量:77
标识
DOI:10.1016/j.phymed.2020.153458
摘要

Ulcerative colitis (UC) is a chronic inflammatory bowel disease with high morbidity, which leads to poor quality of life. The Xianglian pill (XLP) is a classical Chinese patent medicine and has been clinically proven to be an effective treatment for UC. The pharmacological mechanism of the key bioactive ingredients of XLP for the treatment of UC was investigated by a network pharmacology and pharmacokinetics integrated strategy. Network pharmacology was used to analyze the treatment effect of nine quantified XLP ingredients on UC. Key pathways were enriched and analyzed by protein-protein interaction and Kyoto Encyclopedia of Genes and Genomes analyses. The effect of XLP on Th17 cell differentiation was validated using a mouse model of UC. The binding of nine compounds with JAk2, STAT3, HIF-1α, and HSP90AB1 was assessed using molecular docking. A simple and reliable ultra-high-performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous quantification of nine ingredients from XLP in plasma and applied to a pharmacokinetic study following oral administration. Nine compounds of XLP, including coptisine, berberine, magnoflorine,berberrubine, jatrorrhizine, palmatine, evodiamine, rutaecarpine, and dehydrocostus lactone, were detected. Network pharmacology revealed 50 crossover genes between the nine compoundsand UC. XLP treats UC mainly by regulating key pathways of the immune system, including Th17 cell differentiation, Jak-Stat, and PI3K-Akt signaling pathways. An in vivo validation in mice found that XLP inhibits Th17 cell differentiation by suppressing the Jak2-Stat3 pathway, which alleviates mucosal inflammation in UC. Molecular docking confirmed that eight compounds are capable of binding with JAk2, HIF-1α, and HSP90AB1, further confirming the inhibitory effect of XLP on the Jak2-Stat3 pathway. Moreover, apharmacokinetic study revealed that the nine ingredients of XLP are exposed in the plasma and colon tissue, which demonstrates its pharmacological effect on UC. This study evaluates the clinical treatment efficacy of XLP for UC. The network pharmacology and pharmacokinetics integrated strategy evaluation paradigm is efficient in discovering the key pharmacological mechanism of herbal formulae.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
keyword完成签到,获得积分10
3秒前
yema完成签到 ,获得积分10
13秒前
sadascaqwqw完成签到 ,获得积分10
13秒前
害羞龙猫完成签到 ,获得积分10
18秒前
JamesPei应助科研通管家采纳,获得10
19秒前
22秒前
24秒前
鲜于之玉发布了新的文献求助10
28秒前
皆可发布了新的文献求助10
29秒前
29秒前
bkagyin应助踏实树叶采纳,获得10
36秒前
王健锟完成签到,获得积分10
43秒前
44秒前
47秒前
王健锟发布了新的文献求助10
50秒前
踏实树叶完成签到,获得积分10
52秒前
jerry发布了新的文献求助10
52秒前
56秒前
jerry完成签到,获得积分10
1分钟前
1分钟前
acarbose发布了新的文献求助10
1分钟前
acarbose完成签到,获得积分10
1分钟前
鉴定为学计算学的完成签到,获得积分10
1分钟前
chenlc971125完成签到 ,获得积分10
1分钟前
酷波er应助grace135采纳,获得10
1分钟前
充电宝应助小萌兽采纳,获得10
1分钟前
jiang应助kento采纳,获得100
1分钟前
Rayoo完成签到,获得积分10
1分钟前
Rayoo发布了新的文献求助20
1分钟前
1分钟前
传奇3应助MH采纳,获得10
1分钟前
1分钟前
时尚的归尘完成签到,获得积分10
2分钟前
赘婿应助科研通管家采纳,获得20
2分钟前
Hayat应助科研通管家采纳,获得10
2分钟前
2分钟前
绝世冰淇淋完成签到 ,获得积分10
2分钟前
东郭一斩完成签到,获得积分10
2分钟前
小萌兽发布了新的文献求助10
2分钟前
2分钟前
高分求助中
【请各位用户详细阅读此贴后再求助】科研通的精品贴汇总(请勿应助) 10000
求 5G-Advanced NTN空天地一体化技术 pdf版 500
International Code of Nomenclature for algae, fungi, and plants (Madrid Code) (Regnum Vegetabile) 500
Maritime Applications of Prolonged Casualty Care: Drowning and Hypothermia on an Amphibious Warship 500
Comparison analysis of Apple face ID in iPad Pro 13” with first use of metasurfaces for diffraction vs. iPhone 16 Pro 500
Towards a $2B optical metasurfaces opportunity by 2029: a cornerstone for augmented reality, an incremental innovation for imaging (YINTR24441) 500
Robot-supported joining of reinforcement textiles with one-sided sewing heads 490
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4067195
求助须知:如何正确求助?哪些是违规求助? 3606218
关于积分的说明 11450803
捐赠科研通 3327641
什么是DOI,文献DOI怎么找? 1829475
邀请新用户注册赠送积分活动 899393
科研通“疑难数据库(出版商)”最低求助积分说明 819595