间质细胞
3D生物打印
骨钙素
移植
细胞生物学
细胞外基质
间充质干细胞
骨桥蛋白
骨髓
运行x2
组织工程
骨形态发生蛋白2
化学
体外
材料科学
免疫学
生物医学工程
生物
内科学
医学
成骨细胞
碱性磷酸酶
生物化学
酶
作者
Woo Sub Yang,Won Jin Kim,Ji Yeon Ahn,JiUn Lee,Dong Woo Ko,Sumin Park,Ji Yoon Kim,Chul Ho Jang,Jeong Mook Lim,GeunHyung Kim
标识
DOI:10.1021/acsami.0c13905
摘要
This study examined whether neonatal chicken bone marrow cells (cBMCs) could support the osteogenesis of human stromal cells in a three-dimensional (3D) extracellular bioprinting niche. The majority (>95%) of 4-day-old cBMCs subcultured 5 times were positive for osteochondrogenesis-related genes (Col I, Col II, Col X, aggrecan, Sox9, osterix, Bmp2, osteocalcin, Runx2, and osteopontin) and their related proteins (Sox9, collagen type I, and collagen type II). LC–MS/MS analysis demonstrated that cBMC-conditioned medium (c-medium) contained proteins related to bone regeneration, such as periostin and members of the TGF-β family. Next, a significant increase in osteogenesis was detected in three human adipose tissue-derived stromal cell (hASC) lines, after exposure to c-medium concentrates in 2D culture (p < 0.05). To evaluate biological function in a 3D environment, we employed the cBMC-derived bioactive components as a cell-supporting biomaterial in collagen bioink, which was printed to construct a 3D hASC-laden scaffold for observing osteogenesis. Complete osteogenesis was detected in vitro. Moreover, after transplantation of the hASC-laden structure into rats, prominent bone formation was observed compared with that in control rats receiving scaffold-free hASC transplantation. These results demonstrated that substance(s) secreted by chick bone marrow cells clearly activated the osteogenesis of hASCs in 2D- or 3D-niches.
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