Effect of the Exogenous Amino Acid Concentration on the Rate of Gluconeogenesis in Liver Slices

The steady state rate of gluconeogenesis in rat liver slices incubated in U-14C-alanine was linearly related to the extracellular alanine concentration over a range of 1-9 mg of alanine/100 ml. The rate of oxidation of alanine to CO2 was also linearly related to the extracellular alanine concentration and the ratio of alanine oxidized to CO2 to the alanine converted to glucose was constant with changes in the alanine concentration.