Production Of 2, 5-Hexanediol Using Pichia farinose: A Response Surface Methodology

Hexanediol is being used in perfume, medicine and as an intermediate in the preparation of optically active tetrahydrofurans used in biodegradable polymers. Commercially hexanediol is produced chemically which is a very cost effective wi th very low productivity. In this project we have tried to produce Hexanediol using Pichia farinose MTCC*246 (a yeast) and have optimized the process for batch production. Box -Beckmen method of Response Surface Methodology (Minitab14 ® Software) was used for the optimization of temperature, pH, glucose and hexanedione concentration (substrate). The final product Hexanediol in fermentation broth was extracted using ethyl acetate and estimated using Gas chromatography (Perkin Elmer USA). The optimum temperature, pH, glucose, substrate concentration and time for the gr owth of micro-organism and Hexanediol production were found to be 33.038˚C, 7.53, 55g/l, 5.5mM and 48hrs respectively.