Cultural characteristics of Fusarium oxysporum f. sp. cubense race 4 on culture media

The selectivity and identification of K2,PPA,MBA and PSA media for Fusarium oxysporum f.sp.cubense race 4 were compared.The results showed that K2 media allowed the development of F.oxysporum f.sp.cubense race 4 and formed the laciniated colonies,whereas contaminants were greatly suppressed.The strain,isolated on K2 media from natural soil,was identified as F.oxysporum f.sp.cubense race 4 by pathogenicity test.Therefore,the media is a potent differential media for isolation of F.oxysporum f.sp.cubense race 4,which is convenient for detecting F.oxysporum f.sp.cubense race 4 from soil,seedling and host tissue.