Isolation and expression analysis of BRANCHED1 in Dendranthema grandiflora 'Jinba'.

A BRANCHED1-like gene named DgBRC1b was cloned by RT-PCR and rapid amplification of cDNA ends(RACE) using Dendranthema grandiflora 'Jinba'.A predicated 1 008 bp open reading frame was deduced in the cloned sequence,which encoded 335 amino acids.By alignment analysis,the deduced amino acid sequence contained two conserved regions which was identified as diagnostic of true TCP.The gene was proposed to belong to TB1 subfamily by phylogenetic analysis.Subcellular localization revealed that DgBRC1b was localized preferentially distributed to nucleus.