生物传感器
材料科学
纳米结构
分析物
纳米技术
检出限
DNA
纳米颗粒
小RNA
组合化学
化学
色谱法
生物化学
基因
作者
Peng Miao,Baozhen Wang,Xifeng(陈锡峰) Chen,Xiaoxi(李小溪) Li,Ying Tang
标识
DOI:10.1021/acsami.5b01508
摘要
MicroRNAs are not only important regulators of a wide range of cellular processes but are also identified as promising disease biomarkers. Due to the low contents in serum, microRNAs are always difficult to detect accurately . In this study, an electrochemical biosensor for ultrasensitive detection of microRNA based on tetrahedral DNA nanostructure is developed. Four DNA single strands are engineered to form a tetrahedral nanostructure with a pendant stem-loop and modified on a gold electrode surface, which largely enhances the molecular recognition efficiency. Moreover, taking advantage of strand displacement polymerization, catalytic recycling of microRNA, and silver nanoparticle-based solid-state Ag/AgCl reaction, the proposed biosensor exhibits high sensitivity with the limit of detection down to 0.4 fM. This biosensor shows great clinical value and may have practical utility in early diagnosis and prognosis of certain diseases.
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