Determination of Eight Ginsenosides in Ginseng Extracts by UPLC

A method for rapid determination of 8 ginsenosides in ginseng extracts of different sources using ultra performance liquid chromatography(UPLC) was established. The content of ginsenosides in 44 batches of ginseng extracts from 13 sources was analyzed and evaluated. The separation was performed on a Waters ACQUITY UPLC BEH? C 18 column(50 mm×2.1 mm, 1.7 μm) at 30 ℃ at a fl ow rate of 0.5 mL/min through gradient elution with acetonitrile-water mixture and detection at a wavelength of 203 nm. Eight ginsenosides were well separated within 15 min with correlation coeffi cients of 0.9990–0.9997. The detection limits for ginsenosides were 0.47-1.96 ng with recoveries ranging from 95.0% to 110.0%. The method was simple, rapid, and reliable, and thus, was suitable for the rapid determination of 8 ginsenosides in ginseng extracts from different sources.