An in vitro Model for Decidual Reaction of Human Endometrium and the Relationship between Decidualization & Gland

The stromal cells and glands of human endometrium were isolated and cultured. After stro- mal cells had been confluent, they were induced decidualization by P stimuli. The results showed that the stromal cells in the P-treated group became larger and ovoid, especially in the FBS group. The peak PRL level in the group P was obtained at the 17th ~19th d. Immunocy- tochemical staining for PRL showed that the strong immunoreactive signal was obtained in this time group. After establiment in vitro decidualization of stromal cells, the homogenate from glan- dular epithelial cells was added into the decidual cell. The results revealed that the decidual cells became smaller when the homogenate was added for 3 days ,the PRL production in the 1μg/ml group was decreased but the PRL content in the control group was gradually augmented. These findings suggest that the decidualization of stromal cells can be inhibited by the glandular epithe- lial cells, and the pattern and mechanism need to be further studied.