The clinical significance of Y chromosomal microdeletions detection in male infertility

Objective To assess the relevant relationship and clinical significance of Y chromosomal microdeletions and male infertility.Methods Y chromosome microdeletions were detected by 4sets of multiple polymerase chain reactions from 196 male infertility patients with 31 cases azoospermia and165 cases severe oligozoospermia and 30 normal fertile men.15 Y chromosome specific sequence-tagged site labels(STS)distributed in AZFa、AZFb、AZFc、AZFd were amplified.Results Of the 196 cases with azoospermia or severe oligozoospermia,14 cases were found with Y chromosome microdeletions,given the prevalence rate of deletion was 7.14%(14/196).AZFc+d were the most common deletions found in Y chromosome.No such deletion was observed among the 30 normal fertile men in the control group.Among those patients,4 cases(28.57%)with microdeletion in AZFa and 5 cases(35.72%)in AZFc+d regions,were all characterized with severe oligozoospermia;4 cases(28.57%)with Y chromosome microdeletions in AZFb+c+d,1 patient were characterized with azoospermia and the other 3 cases with severe oligozoospermia were detected.1 cases(7.14%)with highly microdeletion inAZFa+b+c+d regions were mainly characterized with azoospermia.Conclusion Y chromosomal microdeletions is one of the major cause for male infertility.Analysis of them will provides science indication to diagnosis and therapy of male infertility.