This paper presents technology conditions of enzymatic hydrolysis of poplar wood powder. The pretreatment conditions are 900 g/L propionic acid, 3 g/L HCl, reaction temperature 121 ℃, reaction time 90 minutes. The best conditions of enzymatic hydrolysis are 50 ℃ of enzymatic hydrolysis temperature, 1.00 mL cellulose per 100 g (substrate), 50 g/L of substrate concentration and 72 hrs of hydrolysis duration.The glucose content may reach 22.5 g/L.