Effect of apocynum venetum etract on angiotensin II-induced myocardial fibrosis

Objective To investigate the influence and the mechanisms involved in the regression of apocynum venetum extract(AVE)on Ang Ⅱ-induced myocardial fibrosis.AVE on myocardial fibrosis.Methods Rat cardiac fibroblasts were pretreated with Ang Ⅱ,and then,treated with different concentrations of AVE(0.8mol·L-1、0.4mol·L-1、0.2mol·L-1).The effect of AVE on the proliferation of cardiac fibroblast stimulated by Ang Ⅱ was detected by MTT,while the expression of Col Ⅰ/Col Ⅲ and TGF-β by ELISA and RT-PCR.Results In the cardiac fibroblast,the over expression of Col Ⅰ and Col Ⅲ,induced by Ang II,could be inhibited by AVE.What's more,compared with C group,TGF-β was increased obviously in the Ang Ⅱ group,and all the AVE groups were able to inhibit the increament of TGF-β stimulated by AngII.Conclusion AVE can reduce the expression of mRNA and protein of TGF-β,and as a result,lighten myocardial fibrosis