Determination of Hyperoside in Suspension Culture of Hypericum perforatum L. Cells by HPLC

Objective To establish a HPLC method for the determination of the content of hyperoside insuspension culture of Hypericum perforatum L.cells.Methods The separation was performed on Hypersil ODS2-C18 column(4.6 50mm,5) with a gradient mobile phase consisting of a mixture of 0.5% phosphoric acid-methanol.The column temperature is 30℃,the flow rate was 1.0 mLin-1 and the detective wavelength was 358 nm.ResultsThe linearity of the method was well within the range from 0.174 to 1.740μg(r=0.999 8) and the average recoveryof hyperoside was 99.25% with RSD=1.28%(n=6).Conclusion The method is simple,accurate and reliable,soit can be used for the quality control of Hypericum perforatum L.cells production in factories.