The effects of resveratrol on transplant rejection and expression of IL-2 and IL-10 in the islet-like cell-cluster transplantation

Objective To study the effects of resveratrol(Res) on anti-graft rejection and expression of relevant cell factors in the islet-like cell clusters(ICCs) transplantation.Methods Twenty-four Kunming mice were randomly assigned into 4 groups(6 each): ciclosporin-A(CsA) positive control group,Res group,CsA+Res group and negative control group.Every mouse was treated with different agent once a day from the 3rd day pre-transplant till the 5th and 10th day after transplantation.The pathological sections were made routinely for the grafts from each group of mice.Some sections were used to observe the immunological rejection.The other sections were immunohistochemically stained to detect the expression of IL-2 and IL-10.Results The graft rejection happened in a varied degree in the four groups.The transplanted cells survived in each group,except in negative control group in which the immunosuppressive agent was not used.The positive rate of IL-2 expression was significantly lower in Res group and CsA+Res group(17.5% and 14.5%,respectively) than in CsA positive control group(59.5%,P0.05),while no significant difference was found between Res group and CsA+Res group on the positive rate of IL-10 expression(P0.05).The positive rate of IL-10 expression was significantly higher in Res group and CsA+Res group(23% and 48% respectively) than in CsA positive control(12.5%,P0.05),and the difference between CsA+Res group and Res group was found to be significant(P0.05).On the 10th day after transplantation,a few transplanted cells survived in CsA+Res group,but no transplanted cells was found in other groups.The expressions of IL-2 and IL-10 in CsA positive control group were reduced obviously,and in other groups IL-2 and IL-10 were barely expressed.Conclusion Res could prevent acute graft rejection in islet-like cell clusters transplantation in mice by up-regulating the IL-2 expression and down-regulating the IL-10 expression.