Anthracnose caused by Colletotrichum gloeosporioides is one of the most damaging disease causing reduction of flower set and yield losses in mango. Application of fungicide was one of the approaches to control the disease. In this study, the efficacy of azoxystrobin, one of the strobilurin class fungicides, was evaluated both in vitro and in vivo conditions. In in vitro tests, azoxystrobin completely inhibited mycelial growth of C. gloeosporioides. In field experiment, azoxystrobin at 1, 2 and 4 ml/l significantly suppressed the development of both panicle and leaf anthracnose. Mango trees treated with azoxystrobin produced more fruits compared to control and showed no phytotoxicity. The reduction of anthracnose incidence and yield increased curve obtain, shows flattening between the range 2.0 and 4.0 ml/l rates, hence the optimum rate of azoxystrobin was fixed to be at 2.0 ml/l for the control of anthracnose disease. This systemic fungicide azoxystrobin was useful in managing this destructive disease of mango in field.