Induction in vitro and stability of Mycobacterium tuberculosis resistance to ofloxacin

Objective To induct Mycobacterium tuberculosis(MTB)resistance with ofloxacin(Ofx)of stepwise increasing concentration in vitro,investigate stability to fluoroquinolone(FQs)antibiotic of MTB,and analyze the molecular mechanism and mutation specialty of drug resistance preliminarily.Methods MTB Standard strain