光滑假丝酵母
氟康唑
生物
基因组DNA
微生物学
基因组
聚合酶链反应
DNA
抗真菌
基因
遗传学
作者
Oscar Hernández-Carreón,Cesia Hernández-Howell,Grecia Hernández-Hernández,M. Selene Herrera-Basurto,Blanca E. González-Gómez,Guadalupe Gutiérrez-Escobedo,Norma Eugenia García-Calderón,Daniel Barrón-Pastor,Alejandro De Las Peñas,Irene Castaño
标识
DOI:10.1007/s42770-021-00584-2
摘要
The most common nosocomial fungal infections are caused by several species of Candida, of which Candida glabrata is the second most frequently isolated species from bloodstream infections. C. glabrata displays relatively high minimal inhibitory concentration values (MIC) to the antifungal fluconazole and is associated with high mortality rates. To decrease mortality rates, the appropriate treatment must be administered promptly. C. glabrata contains in its genome several non-identical copies of species-specific sequences. We designed three pairs of C. glabrata-specific primers for endpoint PCR amplification that align to these species-specific sequences and amplify the different copies in the genome. Using these primers, we developed a fast, sensitive, inexpensive, and highly specific PCR-based method to positively detect C. glabrata DNA in a concentration-dependent manner from mixes of purified genomic DNA of several Candida species, as well as from hemocultures and urine clinical samples. This tool can be used for positive identification of C. glabrata in the clinic.
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