橄榄油
RNA干扰
生物
基因沉默
基因表达
脂氧合酶
基因
功能基因组学
生物化学
植物
酶
基因组
核糖核酸
基因组学
作者
Sergio Cerezo,M. Luisa Hernández,Elena Palomo-Ríos,Naima Gouffi,Lourdes García-Vico,M. Dolores Sicardo,Carlos Sanz,José A. Mercado,Fernando Pliego‐Alfaro,José M. Martínez‐Rivas
出处
期刊:Plant Science
[Elsevier]
日期:2021-12-01
卷期号:313: 111083-111083
被引量:9
标识
DOI:10.1016/j.plantsci.2021.111083
摘要
The C6 aldehydes, alcohols, and the corresponding esters are the most important compounds of virgin olive oil aroma. These C6 volatile compounds are synthesized via the 13-hydroperoxide lyase (13-HPL) branch of the lipoxygenase pathway. In this investigation, a functional analysis of the olive (Olea europaea L.) 13-HPL gene by its overexpression and silencing in olive transgenic lines was carried out. With this aim, sense and RNAi constructs of the olive 13-HPL gene were generated and used for the transformation of embryogenic olive cultures. Leaves from overexpressing lines showed a slight increase in 13-HPL gene expression, whereas RNAi lines exhibited a strong decrease in their transcript levels. Quantification of 13-HPL activity in two overexpressing and two RNAi lines showed a positive correlation with levels of transcripts. Interestingly, RNAi lines showed a high decrease in the content of C6 volatiles linked to a strong increase of C5 volatile compounds, altering the volatile profile in the leaves. In addition, the silencing of the 13-HPL gene severely affected plant growth and development. This investigation demonstrates the role of the 13-HPL gene in the biogenesis of olive volatile compounds and constitutes a functional genomics study in olive related to virgin olive oil quality.
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