Fish spermatozoa are general immotile in the testis and sperm plasma, motility is induced after the discharge of sperm into the aqueous environment.The motility are influence by osmolality,ion,CO 2 and pH value. The environmental factors which activate cAMP ATP Mg 2+ system will affect spermatozoa motility. Motility of spermatozoa were measured by the duration of their movement, the percentage of motile sperm, their velocity and flagella beat frequency. The changes of sperm motility in the genital tract of fishes are also been discussed..