生物
结核分枝杆菌
质粒
转化（遗传学）
转座因子
电穿孔
遗传学
基因组DNA
DNA
突变
核酸
转座子突变
计算生物学
肺结核
突变
基因组
基因
病理
医学
作者
Michelle H. Larsen,Karolin Biermann,Steven Tandberg,Tsugunda Hsu,William R. Jacobs
标识
DOI：10.1002/9780471729259.mc10a02s6
摘要
Abstract This unit includes protocols for the genetic manipulation of Mycobacterium tuberculosis , including nucleic acid extraction (plasmid DNA, genomic DNA, and mRNA), and methods for electroporation (transformation), transduction (including allelic exchange), and transposon mutagenesis. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed. Curr. Protoc. Microbiol . 6:10A.2.1‐10A.2.21. © 2007 by John Wiley & Sons, Inc.
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