Multiplexed Metabolic and Protein Engineering for D‐Limonene Overproduction in Saccharomyces cerevisiae

using a protein scaffold, resulting in 2080.47 mg/L of d-limonene in shake-flask fermentation. Finally, scale-up fed-batch fermentation in a 5 L bioreactor produced 5.96 g/L of d-limonene. Collectively, these results establish a robust and scalable microbial platform for industrial d-limonene production and provide a versatile framework for the biosynthesis of other valuable monoterpenes.