分解代谢抑制
己糖
生物转化
糖
半乳糖
大肠杆菌
生物化学
分解代谢
化学
甘露糖
食品科学
新陈代谢
酶
发酵
突变体
基因
作者
Todd C. Chappell,Nikhil U. Nair
摘要
Escherichia coli is an important commercial species used for production of biofuels, biopolymers, organic acids, sugar alcohols, and natural compounds. Processed biomass and agroindustrial byproducts serve as low-cost nutrient sources and contain a variety of hexoses available for bioconversion. However, metabolism of hexose mixtures by E. coli is inefficient due to carbon catabolite repression (CCR), where the transport and catabolic activity of one or more carbon sources is repressed and/or inhibited by the transport and catabolism of another carbon source. In this work, we developed a microconsortium of different E. coli strains, each engineered to preferentially catabolize a different hexose-glucose, galactose, or mannose. We modified the specificity and preference of carbon source using a combination of rational strain design and adaptive evolution. The modifications ultimately resulted in strains that preferentially catabolized their specified sugar. Finally, comparative analysis in galactose- and mannose-rich sugar mixtures revealed that the consortium grew faster and to higher cell densities compared to the wild-type strain. Biotechnol. Bioeng. 2017;114: 2309-2318. © 2017 Wiley Periodicals, Inc.
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