化学
DNA甲基化
亚硫酸氢盐
亚硫酸氢盐测序
色谱法
甲基化
同位素稀释
基因组DNA
DNA
串联质谱法
放大器
液相色谱-质谱法
质谱法
照明菌甲基化试验
计算生物学
分子生物学
聚合酶链反应
基因
生物化学
生物
基因表达
作者
Xiaoxia Ye,Lijian Zhang,Bin Chen,Jian Li,Qingjin Yang,Qionglin Huang,Junjie Zhang,Yongping Gao,Zhaojin Li,Chun Cai
出处
期刊:Talanta
[Elsevier]
日期:2017-07-01
卷期号:169: 136-140
被引量:11
标识
DOI:10.1016/j.talanta.2017.03.082
摘要
Aberrant DNA methylation is associated with various diseases. Quantitative analysis of regional DNA methylation levels of some specific genes would aid in diseases diagnosis and risk stratification. In this study, we developed a robust method for detecting DNA methylation level over targeted genomic regions using nucleobases quantification in bisulfite amplicons by isotope dilution liquid chromatography tandem mass spectrometry coupled with a simple equation. This method had wide detection range (from 0% to 100% methylation) and high accuracy while more time-saving compared to clonal bisulfite sequencing method. The application for clinical tissue samples showed good applicability and cost effectiveness. This analytical method is suitable for quantifying average DNA methylation level over targeted genomic regions and expected to be a useful tool for detecting DNA methylation biomarkers.
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