Spiro Meroterpenoids from Ganoderma applanatum

Spiroapplanatumines A–Q ( 1 – 12, 14 – 16, 18, and 20 ), new spiro meroterpenoids respectively bearing a 6/5/7 or 6/5/5 ring system, along with three known compounds, spirolingzhines A, B, and D, were isolated from the fruiting bodies of the fungus Ganoderma applanatum . Their structures including absolute configurations were assigned by using spectroscopic methods, ECD and 13 C NMR calculations, and single-crystal X-ray diffraction analysis. Biological evaluation of all the compounds disclosed that compounds 7 and 8 inhibited JAK3 kinase with IC 50 values of 7.0 ± 3.2 and 34.8 ± 21.1 μM, respectively.