噬菌体MS2
生物
噬菌体
先天免疫系统
大肠杆菌
干扰素
RNA沉默
TLR3型
微生物学
病毒学
免疫系统
核糖核酸
RNA干扰
基因
Toll样受体
免疫学
遗传学
作者
Han Lu,Xinjie Guo,Cunshuan Xu,Wenlong Shen,Zhihu Zhao
标识
DOI:10.1016/j.bbrc.2024.149915
摘要
Viral infections pose a significant threat to public health, and the production of interferons represents one of the most critical antiviral innate immune responses of the host. Consequently, the screening and identification of compounds or reagents that induce interferon production are of paramount importance. This study commenced with the cultivation of host bacterium 15597, followed by the infection of Escherichia coli with the MS2 bacteriophage. Utilizing the J2 capture technique, a class of dsRNA mixtures (MS2+15597) was isolated from the E. coli infected with the MS2 bacteriophage. Subsequent investigations were conducted on the immunostimulatory activity of the MS2+15597 mixture. The results indicated that the dsRNA mixtures (MS2+15597) extracted from E. coli infected with the MS2 bacteriophage possess the capability to activate innate immunity, thereby inducing the production of interferon-β. These dsRNA mixtures can activate the RIG-I and TLR3 pattern recognition receptors, stimulating the expression of interferon stimulatory factors 3/7, which in turn triggers the NF-κB signaling pathway, culminating in the cellular production of interferon-β to achieve antiviral effects. This study offers novel insights and strategies for the development of broad-spectrum antiviral drugs, potentially providing new modalities for future antiviral therapies.
科研通智能强力驱动
Strongly Powered by AbleSci AI