衍生化
甘油酯
化学
色谱法
有机化学
高效液相色谱法
脂肪酸
作者
Yuqi Cao,Ying-Jie Lu,Li Zhang,Jing Zhang,Yinlong Guo
标识
DOI:10.1016/j.cclet.2024.109788
摘要
Matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has shown its capability in visualizing the spatial distribution of various kinds of endogenous metabolites. Nevertheless, high quality mass imaging of low polar metabolites remains challenging. Herein, a platform for sensitive matrix-assisted laser desorption ionization-mass spectrometry imaging of cholesterol and glycerides has been proposed. In the platform, a vacuum promoted on-tissue derivatization strategy was proposed to constantly make the derivatization reaction proceed towards to the direction of products. Compared with traditional on-tissue derivatization procedure, the strategy improved the acquired intensity of derivatized glycerides about 50%. Additionally, the mass spectrometry image reflecting the signal ratio between 3 classes of glycerides was achieved to exploit the metabolic level of glycerides on tissue slice. Finally, the platform was applied to brain slices of Alzheimer's transgenic mice, type 2 diabetes mice and normal mice. Significant difference was found in mass spectrometry images reflecting the signal ratio of multiple endogenous metabolites. The work constructed a promising platform for mapping of glycerides in tissue by mass spectrometry imaging.
科研通智能强力驱动
Strongly Powered by AbleSci AI