Sappanumols A–C, Three Homoisoflavonoids with Antiinflammatory Activities from Sappan lignum

Sappanumols A-C (1-3, respectively), three unprecedented homoisoflavonoids, were isolated from Sappan lignum. Their structures were elucidated by comprehensive spectroscopic analysis and ECD computation methods. Compound 3 demonstrated inhibitory effects on LPS-induced NO production in BV-2 cells with an IC₅₀ value of 16.99 μM. Compound 3 decreased the levels of TNF-α and IL-6 in a dose-dependent manner and exhibited significant anti-inflammatory activity in a zebrafish inflammation model.