新生内膜增生
体内
西罗莫司
再狭窄
紫杉醇
涂层
内膜增生
血管内皮生长因子
癌症研究
血管成形术
材料科学
血管生成
治疗性血管生成
内皮
医学
药理学
生物医学工程
支架
内科学
生物
血管内皮生长因子受体
化疗
纳米技术
生物技术
平滑肌
作者
Yu-Xian Lai,Jiandong Fu,Shi Wu,Ren-yun Li,Jiaqi Hu,You‐Xiang Wang,M. Cristina L. Martins,Keke Ren,Guosheng Fu,Jian Ji
摘要
Drug-coated balloon (DCB) is a therapeutic method that can effectively deliver antiproliferative drugs such as paclitaxel and rapamycin (RAPA) with no permanent implants left behind. However, delayed reendothelialization due to the toxicity of the delivered drugs leads to poor therapeutic effects. Here, we propose a new design of DCB coating, which incorporates both vascular endothelial growth factor (VEGF)-encoding plasmid DNA (pDNA) that can promote endothelial repair and RAPA into protamine sulfate (PrS). We demonstrate that the PrS/pDNA/RAPA coating had stability and good anticoagulation properties in vitro. We further show that the coating exhibited excellent transfer capacity from balloon substrates to vessel walls both in vitro and in vivo. Furthermore, the PrS/pDNA/RAPA coating effectively inhibited neointimal hyperplasia after balloon-induced vascular injuries through the down-regulation of the mammalian target of Rapamycin (mTOR) and promoted endothelium regeneration through increased expression of VEGF in vivo. These data indicate that our nanocomposite coating has great potential for use as a novel coating of DCB to treat neointimal hyperplasia after vascular injuries.
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