Humulus lupulus L.: Evaluation of Phytochemical Profile and Activation of Bitter Taste Receptors to Regulate Appetite and Satiety in Intestinal Secretin Tumor Cell Line (STC‐1 Cells)

Scope Inflorescences of the female hop plant ( Humulus lupulus L.) contain biologically active compounds, most of which have a bitter taste. Given the rising global obesity rates, there is much increasing interest in bitter taste receptors (TAS2Rs). Intestinal TAS2Rs can have beneficial effects on obesity when activated by bitter agonists. This study aims to investigate the mechanism of action of a hydroalcoholic hop extract in promoting hormone secretion that reduces the sense of hunger at the intestinal level through the interaction with TAS2Rs. Methods and results The results demonstrate that the hop extract is a rich source of bitter compounds (mainly α‐, β‐acids) that stimulate the secretion of anorexigenic peptides (glucagon‐like peptide 1 [GLP‐1], cholecystokinin [CCK]) in a calcium‐dependent manner while reducing levels of hunger‐related hormones like ghrelin. This effect is mediated through interaction with TAS2Rs, particularly Tas2r138 and Tas2r120 , and through the activation of downstream signaling cascades. Knockdown of these receptors using siRNA transfection and inhibition of Trpm5 , Plcβ‐2 , and other calcium channels significantly reduces the hop‐induced calcium response as well as GLP‐1 and CCK secretion. Conclusions This study provides a potential application of H. lupulus extract for the formulation of food supplements with satiating activity capable of preventing or combating obesity.