Separation and characterization of degradation impurities of upadacitinib by liquid chromatography and high resolution mass spectrometry

Upadacitinib is an oral Janus Kinase inhibitor used for the treatment of rheumatoid arthritis. This research focuses on the forced degradation study of upadacitinib and the characterization of its degradation impurities. Upadacitinib was subjected to various degradation conditions such as hydrolysis (acid, base, neutral), oxidation, thermal, and photolysis according to International Council for Harmonisation guidelines. Twelve degradation impurities of upadacitinib were observed under oxidation (H