Widespread Increase in Enhancer—Promoter Interactions during Developmental Enhancer Activation in Mammals

增强子 染色质 增强子rna 生物 发起人 增强剂陷阱 基因 基因表达调控 转录因子 细胞生物学 遗传学 CTCF公司 基因表达
作者
Zhuoxin Chen,Valentina Snetkova,Grace Bower,Sandra Jacinto,Benjamin Clock,Iros Barozzi,Brandon J. Mannion,Ana Alcaina‐Caro,Javier López-Rı́os,Diane E. Dickel,Axel Visel,L Pennacchio,Evgeny Z. Kvon
标识
DOI:10.1101/2022.11.18.516017
摘要

Abstract Remote enhancers are thought to interact with their target promoters via physical proximity, yet the importance of this proximity for enhancer function remains unclear. Although some enhancer–promoter loops are cell-type-specific, others are stable across tissues or even display reduced physical proximity upon activation. A major challenge is that relatively few enhancers are functionally characterized in vivo , making it difficult to draw generalized conclusions about enhancer–promoter looping during developmental gene activation. Here, we investigate the 3D conformation of enhancers during mammalian development by generating high-resolution tissue-resolved contact maps for nearly a thousand mammalian enhancers with known in vivo activities in ten murine embryonic tissues. We performed enhancer knockouts in mice, which validated newly identified enhancer–promoter chromatin interactions. The majority of enhancers display tissue-specific 3D conformations, and both enhancer–promoter and enhancer–enhancer interactions are significantly stronger upon enhancer activation in vivo . Less than 14% of enhancer–promoter interactions form stably across tissues; however, corresponding enhancers still display highly-tissue-specific activities indicating that their activity is uncoupled from a chromatin interaction with promoters. We find that these invariant interactions form in the absence of the enhancer and are mediated by adjacent CTCF binding. We also identified putative in vivo target genes for enhancers linked to congenital malformations, neurodevelopmental disorders, and autism, demonstrating the utility of our dataset for understanding human congenital disorders. Our results highlight the general significance of enhancer–promoter physical proximity for developmental gene activation in mammals.
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