生物催化
辣根过氧化物酶
化学
壳聚糖
葡萄糖氧化酶
固定化酶
核化学
色谱法
有机化学
催化作用
离子液体
酶
作者
Yaohua Gu,Lin Yuan,Mingming Li,Xinyu Wang,Deyu Rao,Xiaoyan Bai,Keren Shi,Haiming Xu,Shaozhang Hou,Huiqin Yao
出处
期刊:RSC Advances
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:12 (35): 23006-23016
被引量:10
摘要
Co-immobilized bienzyme biocatalysts are attracting increasing interest in the field of wastewater treatment due to their widespread application. In this study, we successfully prepared a co-immobilized bienzyme biocatalyst by immobilizing horseradish peroxidase (HRP) and glucose oxidase (GOD) on dopamine (DA) modified cellulose (Ce)-chitosan (Cs) composite beads via covalent binding, designated as Ce-Cs@DA/HRP-GOD beads, and found that the bienzyme biocatalyst had a good ability to catalytically degrade acridine in wastewater. SEM, EPR, FTIR, and XRD were used to characterise the structure and properties of the Ce-Cs@DA/HRP-GOD beads. The co-immobilized bienzyme biocatalyst with a small amount of HRP exhibited better degradation efficiency for acridine (99.5%, 8 h) in simulated wastewater compared to the Ce-Cs@DA/HRP (93.8%, 8 h) and Ce-Cs@DA/GOD (15.8%, 8 h) beads alone. In addition, a reusability study showed that the co-immobilized bienzyme biocatalyst maintained a degradation rate of 61.2% after six cycles of acridine degradation. The good biodegradability and reusability of the biocatalyst might be due to the synergistic effect of bienzyme HRP-GOD, including the strong covalent bonding. Accordingly, the co-immobilized bienzyme biocatalyst based on the cascade reaction may pave the way for efficient and eco-friendly treatment of industrial wastewater.
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