Comparison between Polymerase Chain Reaction and Loop MediatedIsothermal Amplification for the detection of Staphylococcus aureus in food

This research aimed to compare Polymerase Chain Reaction (PCR) and Loop Mediated Isothermal Amplification (LAMP) for the detection of Staphylococcus aureus in food. Staphylococcus aureus is a human pathogen due to widespread infection and being methicillin-resistant causing bacteraemia and tissue infections. According to six distinct sequences of the mecA and femA in S. aureus, four specific primers that were designed to detect S. aureus using LAMP. In addition, the sensitivity of LAMP was performed and compared with PCR. The results showed that the LAMP completed the reaction within 30 mins at 65°C. LAMP is considered a promising alternative method compared to the PCR technique where LAMP can be used in laboratories and fields with limited resources.