细胞因子
炎症
促炎细胞因子
肽
细胞毒性
药理学
免疫学
生物
化学
生物化学
体外
作者
Kellen Cristina Torres Costa,Vanessa Santana Vieira Santos,Emília Rezende Vaz,Sarah Natalie Cirilo Gimenes,L. E. Corrêa,Jessica Brito de Souza,Francisco Abad Santos,Veridiana de Melo Rodrigues Ávila,Luiz Ricardo Goulart,Vivian Alonso-Goulart
出处
期刊:Toxicon
[Elsevier]
日期:2023-08-01
卷期号:231: 107207-107207
标识
DOI:10.1016/j.toxicon.2023.107207
摘要
Phospholipases A2 (PLA2s) are associated with inflammatory response, performing a complex process involving, specially, cytokines. The excess of pro-inflammatory cytokines induces a chronic inflammatory response and can cause several disorders in the body. Therefore, the inhibition or regulation of cytokines' signaling pathways is a target for new treatment development strategies. Thus, this study aimed to select PLA2 inhibitor mimetic peptides through phage display technology with anti-inflammatory activity. Specific mimetic peptides were selected using BpPLA2-TXI, a PLA2 isolated from Bothrops pauloensis, as a target, and γCdcPL, a PLA2 inhibitor isolated from Crotalus durissus collilineatus, which was used as a competitor during the elution step. We selected the peptide C2PD, which seems to play a pivotal role in the modulation of IL-6, IL-1β, and IL-10 cytokines in inflammatory cells. The C2PD showed a significant reduction in PLA2 activity. Furthermore, the synthetic peptide was tested in PBMC and showed a significant down-modulation of IL-6 and IL-1β release, whereas IL-10 responses were up-regulated. Our findings suggest that this novel peptide may be a potential therapeutic candidate for the treatment of inflammatory diseases, mainly due to its anti-inflammatory properties and absence of cytotoxicity.
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